验证数据展示
经过测试的应用
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60108-1-Ig targets Fibromodulin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Fibromodulin fusion protein Ag4030 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fibromodulin |
| 别名 | fibromodulin, FM, FMOD, KSPG fibromodulin, SLRR2E |
| 计算分子量 | 376 aa, 43 kDa |
| GenBank蛋白编号 | BC035281 |
| 基因名称 | Fibromodulin |
| Gene ID (NCBI) | 2331 |
| RRID | AB_2105538 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | Q06828 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibromodulin antibody 60108-1-Ig | Download protocol |
| IHC protocol for Fibromodulin antibody 60108-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution | ||
J Clin Invest Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment.
| ||
Acta Biomater In situ regeneration of bone-to-tendon structures: Comparisons between costal-cartilage derived stem cells and BMSCs in the rat model. | ||
FASEB J Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential. | ||
BMC Cancer Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis | ||
Cartilage Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel |
