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  • Featured Product
  • KD/KO Validated

FMR1 Monoclonal antibody, PBS Only

FMR1 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB,IP,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

2B5B5

Cat No : 66548-1-PBS

Print datasheet

Synonyms

FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1

验证数据展示

  • WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

    WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66548-1-Ig (FMR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

    IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

    IP result of anti-FMR1 (IP:66548-1-Ig, 5ug; Detection:66548-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

    IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)

    FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

产品信息

66548-1-PBS targets FMR1 in WB, IP, FC, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,FC Application Description
Tested Reactivity Human, rat, mouse
Immunogen FMR1 fusion protein Ag4697 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fragile X mental retardation 1
Synonyms FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 69-72 kDa
GenBank Accession NumberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06787
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.