验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSCT6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue, human liver tissue, human colon cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66548-1-Ig targets FMR1 in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | FMR1 fusion protein Ag4697 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | fragile X mental retardation 1 |
| Synonyms | FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1, FMRP |
| Calculated Molecular Weight | 632 aa, 71 kDa |
| Observed Molecular Weight | 69-72 kDa |
| GenBank Accession Number | BC038998 |
| Gene Symbol | FMR1 |
| Gene ID (NCBI) | 2332 |
| RRID | AB_2881910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q06787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FMR1 antibody 66548-1-Ig | Download protocol |
| IHC protocol for FMR1 antibody 66548-1-Ig | Download protocol |
| IP protocol for FMR1 antibody 66548-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences.
| ||
Environ Res Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease | ||
Front Mol Neurosci Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein |
