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  • KD/KO Validated

FMR1 Monoclonal antibody

FMR1 Monoclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2B5B5

Cat no : 66548-1-Ig

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Synonyms

FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1, FMRP



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSCT6 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, human liver tissue, human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66548-1-Ig targets FMR1 in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FMR1 fusion protein Ag4697 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fragile X mental retardation 1
Synonyms FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1, FMRP
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 69-72 kDa
GenBank Accession NumberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
RRIDAB_2881910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

实验方案

Product Specific Protocols
WB protocol for FMR1 antibody 66548-1-IgDownload protocol
IHC protocol for FMR1 antibody 66548-1-IgDownload protocol
IP protocol for FMR1 antibody 66548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences.

Authors - Raeann Goering
  • KO Validated
mouseWB

Environ Res

Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease

Authors - Belinda J Petri
mouseIF

Front Mol Neurosci

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein

Authors - Cheng Wu