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验证数据展示

  • FC experiment of Jurkat using CL488-66548

    FC experiment of Jurkat using CL488-66548

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FMR1 (CL488-66548, Clone:2B5B5) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FMR1 Monoclonal antibody

FMR1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66548

产品说明书

CloneNo. 2B5B5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, mouse

应用

FC (Intra)

别名

FMR1, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA, POF, POF1, Protein FMR 1, 2B5B5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66548-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66548 targets FMR1 in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat, mouse
免疫原 FMR1 fusion protein Ag4697 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fragile X mental retardation 1
别名 FMR1, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA, POF, POF1, Protein FMR 1, 2B5B5
计算分子量 632 aa, 71 kDa
观测分子量 69-72 kDa
GenBank蛋白编号BC038998
基因名称 FMR1
Gene ID (NCBI) 2332
RRIDAB_3084257
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06787
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FMR1 antibody CL488-66548Download protocol
Standard Protocols
Click here to view our Standard Protocols