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验证数据展示

  • FC experiment of Jurkat using CL488-66548

    FC experiment of Jurkat using CL488-66548

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FMR1 (CL488-66548, Clone:2B5B5) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FMR1 Monoclonal antibody

FMR1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66548

Print datasheet

CloneNo. 2B5B5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Synonyms

FMR1, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA, POF, POF1, Protein FMR 1, 2B5B5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66548-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66548 targets FMR1 in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rat, mouse
Immunogen FMR1 fusion protein Ag4697 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fragile X mental retardation 1
Synonyms FMR1, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA, POF, POF1, Protein FMR 1, 2B5B5
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 69-72 kDa
GenBank Accession NumberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
RRIDAB_3084257
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06787
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FMR1 antibody CL488-66548Download protocol
Standard Protocols
Click here to view our Standard Protocols