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  • Featured Product
  • KD/KO Validated

FNTB Monoclonal antibody, PBS Only

FNTB Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F3B10

Cat no : 66783-1-PBS

Print datasheet

Synonyms

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

验证数据展示

  • WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

    WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66783-1-Ig (same clone as 66783-1-PBS)

    WB analysis of HeLa using 66783-1-Ig (same clone as 66783-1-PBS)

    WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    IF Staining of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    FC experiment of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.8 ug FNTB Monoclonal antibody (66783-1-Ig, Clone:1F3B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

产品信息

66783-1-PBS targets FNTB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen FNTB fusion protein Ag27796 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name farnesyltransferase, CAAX box, beta
Synonyms FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10
Calculated Molecular Weight 437 aa, 49 kDa
Observed Molecular Weight 44-45 kDa
GenBank Accession NumberBC020232
Gene Symbol FNTB
Gene ID (NCBI) 2342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49356
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.