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Host
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

    WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66783-1-Ig (same clone as 66783-1-PBS)

    WB analysis of HeLa using 66783-1-Ig (same clone as 66783-1-PBS)

    WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    IF Staining of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    FC experiment of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.8 ug FNTB Monoclonal antibody (66783-1-Ig, Clone:1F3B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FNTB Monoclonal antibody, PBS Only

FNTB Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66783-1-PBS

产品说明书

CloneNo. 1F3B10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66783-1-PBS targets FNTB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 FNTB fusion protein Ag27796 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 farnesyltransferase, CAAX box, beta
别名 FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10
计算分子量 437 aa, 49 kDa
观测分子量 44-45 kDa
GenBank蛋白编号BC020232
基因名称 FNTB
Gene ID (NCBI) 2342
RRIDAB_2882128
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49356
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.