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验证数据展示

  • FC experiment of A431 using CL594-66783

    FC experiment of A431 using CL594-66783

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human FNTB (CL594-66783, Clone:1F3B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL594-66783

    IF Staining of A431 using CL594-66783

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

  • IF Staining of A431 using CL594-66783

    IF Staining of A431 using CL594-66783

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated FNTB Monoclonal antibody

FNTB Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66783

产品说明书

CloneNo. 1F3B10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66783-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66783 targets FNTB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 FNTB fusion protein Ag27796 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 farnesyltransferase, CAAX box, beta
别名 FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10
计算分子量 437 aa, 49 kDa
观测分子量 44-45 kDa
GenBank蛋白编号BC020232
基因名称 FNTB
Gene ID (NCBI) 2342
RRIDAB_3084726
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49356
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

实验方案

Product Specific Protocols
IF protocol for CL594 FNTB antibody CL594-66783Download protocol
FC protocol for CL594 FNTB antibody CL594-66783Download protocol
Standard Protocols
Click here to view our Standard Protocols