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FOLR1 Monoclonal antibody

FOLR1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4B7

Cat no : 60307-1-Ig

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Synonyms

Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18



经过测试的应用

Positive WB detected inhuman milk, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60307-1-Ig targets FOLR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FOLR1 fusion protein Ag19959 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name folate receptor 1 (adult)
Synonyms Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18
Calculated Molecular Weight 257 aa, 30 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC002947
Gene Symbol FOLR1
Gene ID (NCBI) 2348
RRIDAB_2881421
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15328
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).

实验方案

Product Specific Protocols
WB protocol for FOLR1 antibody 60307-1-IgDownload protocol
IHC protocol for FOLR1 antibody 60307-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Nano

High-Performance Self-Cascade Pyrite Nanozymes for Apoptosis-Ferroptosis Synergistic Tumor Therapy.

Authors - Xiangqin Meng
humanWB

Biosensors (Basel)

Folic Acid-Modified Fluorescent-Magnetic Nanoparticles for Efficient Isolation and Identification of Circulating Tumor Cells in Ovarian Cancer.

Authors - Yue Pan
humanWB

Antibodies (Basel)

Novel Anti-FOLR1 Antibody-Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells.

Authors - Yuki Matsunaga
  • KD Validated