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验证数据展示

  • IF Staining of HepG2 using CL488-66976

    IF Staining of HepG2 using CL488-66976

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 FOX2/RBM9 antibody (CL488-66976, Clone: 2D7G5 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FOX2/RBM9 Monoclonal antibody

FOX2/RBM9 Monoclonal Antibody for IF/ICC
Cat No. CL488-66976

产品说明书

CloneNo. 2D7G5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

IF/ICC

别名

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, fxh, HNRBP2, HRNBP2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66976-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66976 targets FOX2/RBM9 in IF/ICC applications and shows reactivity with Human, Mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse
免疫原 FOX2/RBM9 fusion protein Ag28336 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 9
别名 dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, fxh, HNRBP2, HRNBP2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA
计算分子量 390 aa, 41 kDa
观测分子量 48 kDa, 50 and 60 kDa
GenBank蛋白编号BC013115
基因名称 RBM9
Gene ID (NCBI) 23543
RRIDAB_2919399
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43251
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FOX2/RBM9 antibody CL488-66976Download protocol
Standard Protocols
Click here to view our Standard Protocols