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- KD/KO Validated
CoraLite® Plus 488-conjugated FOX2/RBM9 Monoclonal antibody
FOX2/RBM9 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D7G5
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66976-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66976 targets FOX2/RBM9 in IF/ICC applications and shows reactivity with Human, Mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, Mouse |
Immunogen | FOX2/RBM9 fusion protein Ag28336 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | RNA binding motif protein 9 |
Synonyms | dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, fxh, HNRBP2, HRNBP2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA |
Calculated Molecular Weight | 390 aa, 41 kDa |
Observed Molecular Weight | 48 kDa, 50 and 60 kDa |
GenBank Accession Number | BC013115 |
Gene Symbol | RBM9 |
Gene ID (NCBI) | 23543 |
RRID | AB_2919399 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O43251 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 FOX2/RBM9 antibody CL488-66976 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |