• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    IF Staining of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84020-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    IF Staining of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84020-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    FC experiment of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXF1 Recombinant antibody (84020-3-RR, Clone:241076C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84020-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84020-3-RR

    Affinity and Kinetic Characterization of 84020-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84020-3-RR against Human FOXF1 were performed. The affinity constant is below 1 pM.

FOXF1 Recombinant antibody, PBS Only

FOXF1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84020-3-PBS

产品说明书

CloneNo. 241076C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

241076C8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84020-3-PBS targets FOXF1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 FOXF1 fusion protein Ag34266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box F1
别名 241076C8
计算分子量40 kDa
观测分子量40 kDa
GenBank蛋白编号NP_001442
基因名称 FOXF1
Gene ID (NCBI) 2294
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ12946
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXF1 (Forkhead box protein F1), also known as FKHL5, It is expected to be located in the nucleus, which is expressed predominantly in lung and placenta. FOXF1, a member of the Forkhead box family of transcription factors, as one of the most highly and consistently upregulated genes in FP-RMS. While it is known that FOXF1 is required for mesodermal differentiation toward endothelial and smooth muscle cell lineages, FOXF1 is not expressed in differentiated skeletal muscle cells, and its role in fusion-positive FP-RMS remains unknown (PMID: 33627785). The molecular weight of FOXF1 is 40 kDa.