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验证数据展示

  • IF Staining of HeLa using 84020-3-RR

    IF Staining of HeLa using 84020-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84020-3-RR

    IF Staining of HeLa using 84020-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84020-3-RR

    FC experiment of HeLa using 84020-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXF1 Recombinant antibody (84020-3-RR, Clone:241076C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84020-3-RR

    Affinity and Kinetic Characterization of 84020-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84020-3-RR against Human FOXF1 were performed. The affinity constant is below 1 pM.

FOXF1 Recombinant antibody

FOXF1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84020-3-RR

产品说明书

CloneNo. 241076C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

241076C8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84020-3-RR targets FOXF1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FOXF1 fusion protein Ag34266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box F1
别名 241076C8
计算分子量40 kDa
观测分子量40 kDa
GenBank蛋白编号NP_001442
基因名称 FOXF1
Gene ID (NCBI) 2294
RRIDAB_3671589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ12946
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXF1 (Forkhead box protein F1), also known as FKHL5, It is expected to be located in the nucleus, which is expressed predominantly in lung and placenta. FOXF1, a member of the Forkhead box family of transcription factors, as one of the most highly and consistently upregulated genes in FP-RMS. While it is known that FOXF1 is required for mesodermal differentiation toward endothelial and smooth muscle cell lineages, FOXF1 is not expressed in differentiated skeletal muscle cells, and its role in fusion-positive FP-RMS remains unknown (PMID: 33627785). The molecular weight of FOXF1 is 40 kDa.

实验方案

Product Specific Protocols
IF protocol for FOXF1 antibody 84020-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols