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验证数据展示

  • IF Staining of HeLa using CL488-84020-3

    IF Staining of HeLa using CL488-84020-3

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FOXF1 antibody (CL488-84020-3, Clone: 241076C8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-84020-3

    FC experiment of HeLa using CL488-84020-3

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Foxf1 Recombinant Antibody (CL488-84020-3, Clone:241076C8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated FOXF1 Recombinant antibody

FOXF1 Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84020-3

产品说明书

CloneNo. 241076C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

241076C8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 84020-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84020-3 targets FOXF1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 FOXF1 fusion protein Ag34266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box F1
别名 241076C8
计算分子量40 kDa
观测分子量40 kDa
GenBank蛋白编号NP_001442
基因名称 FOXF1
Gene ID (NCBI) 2294
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12946
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXF1 (Forkhead box protein F1), also known as FKHL5, It is expected to be located in the nucleus, which is expressed predominantly in lung and placenta. FOXF1, a member of the Forkhead box family of transcription factors, as one of the most highly and consistently upregulated genes in FP-RMS. While it is known that FOXF1 is required for mesodermal differentiation toward endothelial and smooth muscle cell lineages, FOXF1 is not expressed in differentiated skeletal muscle cells, and its role in fusion-positive FP-RMS remains unknown (PMID: 33627785). The molecular weight of FOXF1 is 40 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FOXF1 antibody CL488-84020-3Download protocol
FC protocol for CL Plus 488 FOXF1 antibody CL488-84020-3Download protocol
Standard Protocols
Click here to view our Standard Protocols