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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29338-1-AP (same clone as 29338-1-PBS)

    WB analysis using 29338-1-AP (same clone as 29338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29338-1-AP (FOXK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 29338-1-AP (same clone as 29338-1-PBS)

    IP experiment of K-562 using 29338-1-AP (same clone as 29338-1-PBS)

    IP result of anti-FOXK1 (IP:29338-1-AP, 4ug; Detection:29338-1-AP 1:3000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

    IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

    IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • IF Staining of SW480 using 29338-1-AP (same clone as 29338-1-PBS)

    IF Staining of SW480 using 29338-1-AP (same clone as 29338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using FOXK1 antibody (29338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

FOXK1 Polyclonal antibody, PBS Only

FOXK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 29338-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

FOXK1L, MNF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29338-1-PBS targets FOXK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 FOXK1 fusion protein Ag28820 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box K1
别名 FOXK1L, MNF
计算分子量 75 kDa
观测分子量 90-100 kDa
GenBank蛋白编号NM_001037165
基因名称 FOXK1
Gene ID (NCBI) 221937
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP85037
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).