验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, Jurkat cells |
Positive IHC detected in | mouse thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
66337-1-Ig targets FOXN1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA, FC (Intra) Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | FOXN1 fusion protein Ag18207 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | forkhead box N1 |
Synonyms | Winged-helix transcription factor nude, WHN, RONU, Forkhead box protein N1, forkhead box N1 |
Calculated Molecular Weight | 648 aa, 69 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC146539 |
Gene Symbol | FOXN1 |
Gene ID (NCBI) | 8456 |
RRID | AB_2881717 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O15353 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for FOXN1 antibody 66337-1-Ig | Download protocol |
IHC protocol for FOXN1 antibody 66337-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |