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验证数据展示

  • WB analysis of Raji using 66337-1-Ig (same clone as 66337-1-PBS)

    WB analysis of Raji using 66337-1-Ig (same clone as 66337-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

    IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

    IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 66337-1-Ig (same clone as 66337-1-PBS)

    FC experiment of A549 using 66337-1-Ig (same clone as 66337-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human FOXN1 (66337-1-Ig, Clone:1D8B12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

FOXN1 Monoclonal antibody, PBS Only

FOXN1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66337-1-PBS

产品说明书

CloneNo. 1D8B12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Winged-helix transcription factor nude, WHN, RONU, Forkhead box protein N1, forkhead box N1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66337-1-PBS targets FOXN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FOXN1 fusion protein Ag18207 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 forkhead box N1
别名 Winged-helix transcription factor nude, WHN, RONU, Forkhead box protein N1, forkhead box N1
计算分子量 648 aa, 69 kDa
观测分子量 60 kDa
GenBank蛋白编号BC146539
基因名称 FOXN1
Gene ID (NCBI) 8456
RRIDAB_2881717
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15353
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.