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FOXN2 Recombinant antibody

FOXN2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240896C8

Cat no : 83899-2-RR

Print datasheet

Synonyms

Human T-cell leukemia virus enhancer factor, HTLF, Forkhead box protein N2, forkhead box N2, 240896C8

验证数据展示

  • WB analysis using 83899-2-RR

    WB analysis using 83899-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83899-2-RR (FOXN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83899-2-RR

    Affinity and Kinetic Characterization of 83899-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83899-2-RR against Human FOXN2 were performed. The affinity constant is 2.90 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83899-2-RR is FOXN2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FOXN2 fusion protein Ag5126 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name forkhead box N2
Synonyms Human T-cell leukemia virus enhancer factor, HTLF, Forkhead box protein N2, forkhead box N2, 240896C8
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC063305
Gene Symbol FOXN2
Gene ID (NCBI) 3344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32314
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Forkhead box N2 (FOXN2), a member of the FOX transcription factor family, has been found to play key roles in various types of human cancers. A recent report has indicated that FOXN2 is downregulated and serves as a tumor suppressor in T-cell leukemia. Moreover, downregulation of FOXN2 is closely associated with adverse prognosis in adult glioblastoma multiforme treated with radiotherapy or chemotherapy. (PMID: 30655703)

实验方案

Product Specific Protocols
WB protocol for FOXN2 antibody 83899-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols