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验证数据展示

  • WB analysis using 82997-1-RR (same clone as 82997-1-PBS)

    WB analysis using 82997-1-RR (same clone as 82997-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82997-1-RR (FOXO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 82997-1-RR (same clone as 82997-1-PBS)

    IHC staining of human pancreas cancer using 82997-1-RR (same clone as 82997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82997-1-RR (FOXO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXO1 antibody included
  • IF Staining of HepG2 using 82997-1-RR (same clone as 82997-1-PBS)

    IF Staining of HepG2 using 82997-1-RR (same clone as 82997-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXO1 antibody (82997-1-RR, Clone: 230183D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82997-1-RR (same clone as 82997-1-PBS)

    FC experiment of A431 using 82997-1-RR (same clone as 82997-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FOXO1 Recombinant antibody (82997-1-RR, Clone:230183D1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00024-1

    Cytometric bead array standard curve of MP00024-1

    Cytometric bead array standard curve of MP00024-1, FOXO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82997-1-PBS. Detection antibody: 82997-3-PBS. Standard: Ag13296. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 82997-1-RR

    Affinity and Kinetic Characterization of 82997-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82997-1-RR against Human FOXO1 were performed. The affinity constant is 0.188 nM.

FOXO1 Recombinant antibody, PBS Only (Capture)

FOXO1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 82997-1-PBS

产品说明书

CloneNo. 230183D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.

产品信息

82997-1-PBS targets FOXO1 as part of a matched antibody pair:

MP00024-1: 82997-1-PBS capture and 82997-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 FOXO1 fusion protein Ag13296 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box O1
别名 Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1
计算分子量 655 aa, 70 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC021981
基因名称 FOXO1
Gene ID (NCBI) 2308
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12778
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).