验证数据展示
产品信息
66428-1-PBS targets FOXO3A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, Indirect ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | FOXO3A fusion protein Ag1289 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | forkhead box O3 |
Synonyms | AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A |
Calculated Molecular Weight | 673 aa, 71 kDa |
Observed Molecular Weight | 85-90 kDa |
GenBank Accession Number | BC021224 |
Gene Symbol | FOXO3A |
Gene ID (NCBI) | 2309 |
RRID | AB_2881799 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O43524 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)