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FOXP3 Monoclonal antibody

FOXP3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1E7

Cat no : 68803-2-Ig

Print datasheet

Synonyms

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

验证数据展示

  • IHC staining of human tonsillitis using 68803-2-Ig

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included
  • WB analysis of MJ using 68803-2-Ig

    WB analysis of MJ using 68803-2-Ig

    MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 68803-2-Ig

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included
  • IHC staining of human rectal cancer using 68803-2-Ig

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included
  • IHC staining of human appendicitis using 68803-2-Ig

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP3 antibody included

经过测试的应用

Positive WB detected inMJ cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human lymphoma tissue, human ovary cancer tissue, human rectal cancer tissue, MJ cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FOXP3 Ready-To-Use IHC Kit. FOXP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68803-2-Ig targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FOXP3 fusion protein Ag23089 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name forkhead box P3
Synonyms Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID
Calculated Molecular Weight 431 aa, 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC113401
Gene Symbol FOXP3
Gene ID (NCBI) 50943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BZS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.

实验方案

Product Specific Protocols
WB protocol for FOXP3 antibody 68803-2-IgDownload protocol
IHC protocol for FOXP3 antibody 68803-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols