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验证数据展示

  • IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

    IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXP3 antibody included
  • WB analysis of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

    WB analysis of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

    MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

    IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXP3 antibody included
  • IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

    IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXP3 antibody included
  • IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

    IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXP3 antibody included
  • Cytometric bead array standard curve of MP50216-1

    Cytometric bead array standard curve of MP50216-1

    Cytometric bead array standard curve of MP50216-1, FOXP3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68803-2-PBS. Detection antibody: 68838-1-PBS. Standard:Ag17571. Range: 0.781-100 ng/mL.

FOXP3 Monoclonal antibody, PBS Only (Capture)

FOXP3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 68803-2-PBS

产品说明书

CloneNo. 2A1E7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOXP3 Ready-To-Use IHC Kit. FOXP3 primary antibody included.

产品信息

68803-2-PBS targets FOXP3 as part of a matched antibody pair:

MP50216-1: 68803-2-PBS capture and 68838-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 FOXP3 fusion protein Ag23089 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 forkhead box P3
别名 Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID
计算分子量 431 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC113401
基因名称 FOXP3
Gene ID (NCBI) 50943
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BZS1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.