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验证数据展示

  • IF Staining of MCF-7 using CL594-66263

    IF Staining of MCF-7 using CL594-66263

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66263 (FRS2 antibody) at dilution of 1:100.

CoraLite®594-conjugated FRS2 Monoclonal antibody

FRS2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66263

产品说明书

CloneNo. 4E10H12

宿主/亚型

Mouse / IgG3

种属反应性

human

应用

IF/ICC

别名

FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FRS2 Ready-To-Use IHC Kit. FRS2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66263-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66263 targets FRS2 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 FRS2 fusion protein Ag19299 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor receptor substrate 2
别名 FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1
计算分子量 60 kDa
观测分子量 68 kDa
GenBank蛋白编号BC021562
基因名称 FRS2
Gene ID (NCBI) 10818
RRIDAB_2883546
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WU20
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

实验方案

Product Specific Protocols
IF protocol for CL594 FRS2 antibody CL594-66263Download protocol
Standard Protocols
Click here to view our Standard Protocols