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CoraLite®594-conjugated FRS2 Monoclonal antibody

FRS2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4E10H12

Cat no : CL594-66263

Print datasheet

Synonyms

FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1

验证数据展示

  • IF Staining of MCF-7 using CL594-66263

    IF Staining of MCF-7 using CL594-66263

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66263 (FRS2 antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66263-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66263 targets FRS2 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen FRS2 fusion protein Ag19299 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name fibroblast growth factor receptor substrate 2
Synonyms FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC021562
Gene Symbol FRS2
Gene ID (NCBI) 10818
RRIDAB_2883546
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WU20
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

实验方案

Product Specific Protocols
IF protocol for CL594 FRS2 antibody CL594-66263Download protocol
Standard Protocols
Click here to view our Standard Protocols