验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22665-1-AP targets FSHR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, chicken, bovine, sheep, goat |
| 免疫原 | FSHR fusion protein Ag18158 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | follicle stimulating hormone receptor |
| 别名 | LGR1, FSHRO, FSH-R, FSH R, Follitropin receptor |
| 计算分子量 | 695 aa, 78 kDa |
| 观测分子量 | 71-78 kDa |
| GenBank蛋白编号 | BC118548 |
| 基因名称 | FSHR |
| Gene ID (NCBI) | 2492 |
| RRID | AB_2631204 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23945 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FSHR antibody 22665-1-AP | Download protocol |
| IHC protocol for FSHR antibody 22665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline | ||
Autophagy Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1 | ||
Theranostics FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation | ||
J Adv Res Core fucosylation regulates the ovarian response via FSH receptor during follicular development | ||
Redox Biol Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. | ||
Cell Mol Life Sci P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1 |
