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验证数据展示

  • FC experiment of HeLa using CL488-22665

    FC experiment of HeLa using CL488-22665

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite®488 FSHR Polyclonal Antibody (CL488-22665)(red), or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®488-conjugated FSHR Polyclonal antibody

FSHR Polyclonal Antibody for FC (Intra)
Cat No. CL488-22665

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

LGR1, FSHRO, FSH-R, FSH R, Follitropin receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 22665-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22665 targets FSHR in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 FSHR fusion protein Ag18158 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 follicle stimulating hormone receptor
别名 LGR1, FSHRO, FSH-R, FSH R, Follitropin receptor
计算分子量 695 aa, 78 kDa
观测分子量 71-78 kDa
GenBank蛋白编号BC118548
基因名称 FSHR
Gene ID (NCBI) 2492
RRIDAB_3672748
偶联类型 CoraLite®488 Fluorescent Dye
最大激发/发射波长491 nm / 516 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23945
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FSHR, also named as LGR1, belongs to the G-protein coupled receptor 1 family and FSH/LSH/TSH subfamily. FSHR is a receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1). Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS). This antibody is specific to FSHR.

实验方案

Product Specific Protocols
FC protocol for CL488 FSHR antibody CL488-22665Download protocol
Standard Protocols
Click here to view our Standard Protocols