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验证数据展示

  • WB analysis using 28218-1-AP

    WB analysis using 28218-1-AP

    Human placenta tissues were subjected to SDS PAGE followed by western blot with 28218-1-AP (FSTL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28218-1-AP

    WB analysis of HeLa using 28218-1-AP

    WB result of FSTL3 antibody (28218-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL3 transfected HeLa cells.

  • IHC staining of human urothelial carcinoma using 28218-1-AP

    IHC staining of human urothelial carcinoma using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FSTL3 antibody included
  • IHC staining of human urothelial carcinoma using 28218-1-AP

    IHC staining of human urothelial carcinoma using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FSTL3 antibody included
  • Featured Product
  • KD/KO验证

FSTL3/FLRG Polyclonal antibody

FSTL3/FLRG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28218-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FSTL3 Ready-To-Use IHC Kit. FSTL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28218-1-AP targets FSTL3/FLRG in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 FSTL3/FLRG fusion protein Ag27626 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 follistatin-like 3 (secreted glycoprotein)
别名 FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3
计算分子量 263 aa, 28 kDa
观测分子量28-33 kDa
GenBank蛋白编号BC005839
基因名称 FSTL3
Gene ID (NCBI) 10272
RRIDAB_3086037
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95633
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Follistatin‐like 3 (FSTL3) is a highly conserved 27-39 kDa monomeric glycoprotein belonging to the follistatin protein family and it is primarily expressed in the placenta as well as in the testis, heart, adipose tissue, and pancreas. It is an extracellular inhibitor of the transforming growth factor beta (TGF‐β) family ligands, including activin, myostatin, and bone morphogenetic proteins.

实验方案

Product Specific Protocols
WB protocol for FSTL3/FLRG antibody 28218-1-APDownload protocol
IHC protocol for FSTL3/FLRG antibody 28218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols