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  • Featured Product
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FSTL3/FLRG Polyclonal antibody

FSTL3/FLRG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28218-1-AP

Print datasheet

Synonyms

FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3

验证数据展示

  • WB analysis using 28218-1-AP

    WB analysis using 28218-1-AP

    Human placenta tissues were subjected to SDS PAGE followed by western blot with 28218-1-AP (FSTL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28218-1-AP

    WB analysis of HeLa using 28218-1-AP

    WB result of FSTL3 antibody (28218-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL3 transfected HeLa cells.

  • IHC staining of human urothelial carcinoma using 28218-1-AP

    IHC staining of human urothelial carcinoma using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FSTL3 antibody included
  • IHC staining of human urothelial carcinoma using 28218-1-AP

    IHC staining of human urothelial carcinoma using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FSTL3 antibody included

经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FSTL3 Ready-To-Use IHC Kit. FSTL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28218-1-AP targets FSTL3/FLRG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FSTL3/FLRG fusion protein Ag27626 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name follistatin-like 3 (secreted glycoprotein)
Synonyms FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3
Calculated Molecular Weight 263 aa, 28 kDa
Observed Molecular Weight28-33 kDa
GenBank Accession NumberBC005839
Gene Symbol FSTL3
Gene ID (NCBI) 10272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Follistatin‐like 3 (FSTL3) is a highly conserved 27-39 kDa monomeric glycoprotein belonging to the follistatin protein family and it is primarily expressed in the placenta as well as in the testis, heart, adipose tissue, and pancreas. It is an extracellular inhibitor of the transforming growth factor beta (TGF‐β) family ligands, including activin, myostatin, and bone morphogenetic proteins.

实验方案

Product Specific Protocols
WB protocol for FSTL3/FLRG antibody 28218-1-APDownload protocol
IHC protocol for FSTL3/FLRG antibody 28218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols