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验证数据展示

  • WB analysis of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

    WB analysis of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

    WB result of Ferritin heavy chain antibody (83428-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

    IF Staining of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (83428-1-RR, Clone: 230312F3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

  • WB analysis using 83428-1-RR (same clone as 83428-1-PBS)

    WB analysis using 83428-1-RR (same clone as 83428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83428-1-RR

    Affinity and Kinetic Characterization of 83428-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Ferritin heavy chain Recombinant antibody, PBS Only

Ferritin heavy chain Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83428-1-PBS

产品说明书

CloneNo. 230312F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Ferritin, FTH1, 230312F3, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83428-1-PBS targets Ferritin heavy chain in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Ferritin heavy chain fusion protein Ag2290 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ferritin, heavy polypeptide 1
别名 Ferritin, FTH1, 230312F3, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1
计算分子量 183 aa, 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000857
基因名称 FTH1
Gene ID (NCBI) 2495
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP02794
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.