Ferritin heavy chain Recombinant antibody
Ferritin heavy chain Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
230312F3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human liver tissue, human placenta tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83428-1-RR is Ferritin heavy chain Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | Ferritin heavy chain fusion protein Ag2290 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ferritin, heavy polypeptide 1 |
Synonyms | Ferritin H subunit, Ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTH, FTH1, FTHL6, PIG15, PLIF |
Calculated Molecular Weight | 183 aa, 21 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC000857 |
Gene Symbol | FTH1 |
Gene ID (NCBI) | 2495 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P02794 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.
实验方案
Product Specific Protocols | |
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WB protocol for Ferritin heavy chain antibody 83428-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |