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Ferritin heavy chain Recombinant antibody

Ferritin heavy chain Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

230312F3

Cat no : 83428-1-RR

Print datasheet

Synonyms

Ferritin, FTH1, FTH, Ferritin heavy chain, N-terminally processed, EC:1.16.3.1

验证数据展示

  • WB analysis of HeLa using 83428-1-RR

    WB analysis of HeLa using 83428-1-RR

    WB result of Ferritin heavy chain antibody (83428-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells.

  • IF Staining of HeLa using 83428-1-RR

    IF Staining of HeLa using 83428-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (83428-1-RR, Clone: 230312F3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • WB analysis using 83428-1-RR

    WB analysis using 83428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83428-1-RR

    Affinity and Kinetic Characterization of 83428-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, human liver tissue, human placenta tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83428-1-RR is Ferritin heavy chain Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen Ferritin heavy chain fusion protein Ag2290 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ferritin, heavy polypeptide 1
Synonyms Ferritin, FTH1, FTH, Ferritin heavy chain, N-terminally processed, EC:1.16.3.1
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000857
Gene Symbol FTH1
Gene ID (NCBI) 2495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02794
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

实验方案

Product Specific Protocols
WB protocol for Ferritin heavy chain antibody 83428-1-RRDownload protocol
IF protocol for Ferritin heavy chain antibody 83428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Redox Rep

Methane saline suppresses ferroptosis via the Nrf2/HO-1 signaling pathway to ameliorate intestinal ischemia-reperfusion injury

Authors - Qingrui Fan
View Article