验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human liver tissue, human placenta tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
83428-1-RR targets Ferritin heavy chain in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | Ferritin heavy chain fusion protein Ag2290 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ferritin, heavy polypeptide 1 |
| 别名 | Ferritin, FTH1, 230312F3, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1 |
| 计算分子量 | 183 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC000857 |
| 基因名称 | FTH1 |
| Gene ID (NCBI) | 2495 |
| RRID | AB_3671075 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P02794 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Ferritin heavy chain antibody 83428-1-RR | Download protocol |
| IF protocol for Ferritin heavy chain antibody 83428-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Pharm Sci Pharmacodynamic Insights into Maresin 1: Enhancing Flap Viability via the Keap1/Nrf2 Axis to Control ROS-Driven Apoptosis and Ferroptosis | ||
Cancers (Basel) Mitochondrial Iron Metabolism as a Potential Key Mediator of PD-L1 Thermal Regulation |