验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, human placenta tissue |
Positive IHC detected in | mouse brain tissue, human prostate cancer tissue, human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 13 publications below |
IHC | See 1 publications below |
产品信息
68068-1-Ig targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human, Mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | Ferritin light chain fusion protein Ag20637 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ferritin, light polypeptide |
别名 | Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL |
计算分子量 | 19 kDa |
观测分子量 | 18-20 kDa |
GenBank蛋白编号 | BC008439 |
基因名称 | Ferritin light chain |
Gene ID (NCBI) | 2512 |
RRID | AB_2918809 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P02792 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
实验方案
Product Specific Protocols | |
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WB protocol for Ferritin light chain antibody 68068-1-Ig | Download protocol |
IHC protocol for Ferritin light chain antibody 68068-1-Ig | Download protocol |
IF protocol for Ferritin light chain antibody 68068-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Gastroenterol Indoleamine 2,3-dioxygenase 1-mediated iron metabolism in macrophages contributes to lipid deposition in nonalcoholic steatohepatitis | ||
J Neuroimmune Pharmacol Ghrelin Induces Ferroptosis Resistance and M2 Polarization of Microglia to Alleviate Neuroinflammation and Cognitive Impairment in Alzheimer's Disease | ||
Inflammopharmacology Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis | ||
J Nutr Biochem Curcumol ameliorates alcohol and high-fat diet-induced fatty liver disease via modulation of the Ceruloplasmin/iron overload/mtDNA signaling pathway | ||
J Ethnopharmacol Anti-papillary thyroid carcinoma effects of dioscorea bulbifera L. through ferroptosis and the PI3K/AKT pathway based on network pharmacology and experimental validation | ||
Biochim Biophys Acta Mol Basis Dis FTO alleviated ferroptosis in septic cardiomyopathy via mediating the m6A modification of BACH1 |