Ferritin light chain Monoclonal antibody

Ferritin light chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68068-1-Ig

产品说明书

CloneNo. 1F9F5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse and More (3)

应用

WB, IHC, IF/ICC, ELISA

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, human placenta tissue
Positive IHC detected inmouse brain tissue, human prostate cancer tissue, human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FTL Ready-To-Use IHC Kit. FTL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68068-1-Ig targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, Mouse
文献引用反应性human, mouse, rat
免疫原 Ferritin light chain fusion protein Ag20637 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ferritin, light polypeptide
别名 Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL
计算分子量 19 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC008439
基因名称 Ferritin light chain
Gene ID (NCBI) 2512
RRIDAB_2918809
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02792
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

实验方案

Product Specific Protocols
WB protocol for Ferritin light chain antibody 68068-1-IgDownload protocol
IHC protocol for Ferritin light chain antibody 68068-1-IgDownload protocol
IF protocol for Ferritin light chain antibody 68068-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Gastroenterol

Indoleamine 2,3-dioxygenase 1-mediated iron metabolism in macrophages contributes to lipid deposition in nonalcoholic steatohepatitis

Authors - Chaofeng Wu
mouseWB

J Neuroimmune Pharmacol

Ghrelin Induces Ferroptosis Resistance and M2 Polarization of Microglia to Alleviate Neuroinflammation and Cognitive Impairment in Alzheimer's Disease

Authors - Yaoxue Guo
WB

Inflammopharmacology

Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis

Authors - Chenyu Zhao
mouseWB

J Nutr Biochem

Curcumol ameliorates alcohol and high-fat diet-induced fatty liver disease via modulation of the Ceruloplasmin/iron overload/mtDNA signaling pathway

Authors - Tingting Ding
humanWB

J Ethnopharmacol

Anti-papillary thyroid carcinoma effects of dioscorea bulbifera L. through ferroptosis and the PI3K/AKT pathway based on network pharmacology and experimental validation

Authors - Yuzhi Gao
mouseWB

Biochim Biophys Acta Mol Basis Dis

FTO alleviated ferroptosis in septic cardiomyopathy via mediating the m6A modification of BACH1

Authors - Hua Zeng
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