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Ferritin light chain Monoclonal antibody, PBS Only

Ferritin light chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9F5

Cat no : 68068-1-PBS

Print datasheet

Synonyms

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

验证数据展示

  • WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

    WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FTL Ready-To-Use IHC Kit. FTL primary antibody included.

产品信息

68068-1-PBS targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Ferritin light chain fusion protein Ag20637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ferritin, light polypeptide
Synonyms Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC008439
Gene Symbol Ferritin light chain
Gene ID (NCBI) 2512
RRIDAB_2918809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02792
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.