• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68262-1-Ig (same clone as 68262-1-PBS)

    WB analysis using 68262-1-Ig (same clone as 68262-1-PBS)

    Untreated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

    IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FUS/TLS antibody included
  • WB analysis of HeLa using 68262-1-Ig (same clone as 68262-1-PBS)

    WB analysis of HeLa using 68262-1-Ig (same clone as 68262-1-PBS)

    WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; room temperature for 1.5 hours) with negative control and FUS/TLS knockout HeLa cells. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 68262-1-Ig (same clone as 68262-1-PBS)

    IP experiment of K-562 using 68262-1-Ig (same clone as 68262-1-PBS)

    IP result of anti-FUS/TLS (IP:68262-1-Ig, 4ug; Detection:68262-1-Ig 1:5000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

    IF Staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 68262-1-Ig (same clone as 68262-1-PBS)

    FC experiment of HEK-293T using 68262-1-Ig (same clone as 68262-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human FUS/TLS (68262-1-Ig, Clone:1B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FUS/TLS Monoclonal antibody, PBS Only

FUS/TLS Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA
Cat No. 68262-1-PBS

产品说明书

CloneNo. 1B4F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), IP, ELISA

别名

FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy FUS/TLS Ready-To-Use IHC Kit. FUS/TLS primary antibody included.

产品信息

68262-1-PBS targets FUS/TLS in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FUS/TLS fusion protein Ag2150 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fusion (involved in t(12;16) in malignant liposarcoma)
别名 FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein
计算分子量 75 kDa
观测分子量 53 kDa, 68-75 kDa
GenBank蛋白编号BC026062
基因名称 FUS/TLS
Gene ID (NCBI) 2521
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP35637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).