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FUS/TLS Monoclonal antibody

FUS/TLS Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B4F8

Cat no : 68262-1-Ig

Print datasheet

Synonyms

FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein

验证数据展示

  • WB analysis using 68262-1-Ig

    WB analysis using 68262-1-Ig

    Untreated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 68262-1-Ig

    IHC staining of rat brain using 68262-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FUS/TLS antibody included
  • WB analysis of HeLa using 68262-1-Ig

    WB analysis of HeLa using 68262-1-Ig

    WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; room temperature for 1.5 hours) with negative control and FUS/TLS knockout HeLa cells.

  • IF Staining of mouse brain using 68262-1-Ig

    IF Staining of mouse brain using 68262-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HEK-293T using 68262-1-Ig

    FC experiment of HEK-293T using 68262-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human FUS/TLS (68262-1-Ig, Clone:1B4F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, Neuro-2a cells, rat brain tissue, HepG2 cells, HEK-293 cells, mouse brain tissue
Positive IHC detected inrat brain tissue, human ovary tumor tissue, human colon tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy FUS/TLS Ready-To-Use IHC Kit. FUS/TLS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

68262-1-Ig targets FUS/TLS in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FUS/TLS fusion protein Ag2150 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Synonyms FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 53 kDa, 68-75 kDa
GenBank Accession NumberBC026062
Gene Symbol FUS/TLS
Gene ID (NCBI) 2521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).

实验方案

Product Specific Protocols
WB protocol for FUS/TLS antibody 68262-1-IgDownload protocol
IHC protocol for FUS/TLS antibody 68262-1-IgDownload protocol
IF protocol for FUS/TLS antibody 68262-1-IgDownload protocol
FC protocol for FUS/TLS antibody 68262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

Authors - Kaiwen Bao
View Article
humanWB

Arterioscler Thromb Vasc Biol

Exosomes From IgE-Stimulated Mast Cells Aggravate Asthma-Mediated Atherosclerosis Through circRNA CDR1as-Mediated Endothelial Cell Dysfunction in Mice

Authors - Hongqin Yang
View Article
mouseWB

Cell

Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain

Authors - Nicole Scott-Hewitt
View Article