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FUS/TLS Monoclonal antibody
FUS/TLS Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B4F8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, Neuro-2a cells, rat brain tissue, HepG2 cells, HEK-293 cells, mouse brain tissue |
Positive IHC detected in | rat brain tissue, human ovary tumor tissue, human colon tissue, mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue, mouse cerebellum tissue |
Positive FC detected in | HEK-293T cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68262-1-Ig targets FUS/TLS in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC, IF-P, IHC, WB, ELISA |
Tested Reactivity | Human, mouse, rat |
Immunogen | FUS/TLS fusion protein Ag2150 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fusion (involved in t(12;16) in malignant liposarcoma) |
Synonyms | 75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS |
Calculated Molecular Weight | 75 kDa |
Observed Molecular Weight | 53 kDa, 68-75 kDa |
GenBank Accession Number | BC026062 |
Gene Symbol | FUS |
Gene ID (NCBI) | 2521 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P35637 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for FUS/TLS antibody 68262-1-Ig | Download protocol |
IHC protocol for FUS/TLS antibody 68262-1-Ig | Download protocol |
IF protocol for FUS/TLS antibody 68262-1-Ig | Download protocol |
FC protocol for FUS/TLS antibody 68262-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |