验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68262 targets FUS/TLS in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | FUS/TLS fusion protein Ag2150 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fusion (involved in t(12;16) in malignant liposarcoma) |
| 别名 | FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein |
| 计算分子量 | 75 kDa |
| 观测分子量 | 68-75 kDa |
| GenBank蛋白编号 | BC026062 |
| 基因名称 | FUS/TLS |
| Gene ID (NCBI) | 2521 |
| RRID | AB_3084462 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P35637 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FUS/TLS antibody CL488-68262 | Download protocol |
| FC protocol for CL Plus 488 FUS/TLS antibody CL488-68262 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |