验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
10131-1-AP targets FUSIP1/SRS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FUSIP1/SRS10 fusion protein Ag0176 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FUS interacting protein (serine/arginine-rich) 1 |
| 别名 | FUSIP1, Serine/arginine-rich splicing factor 10, NSSR, FUSIP2, FUS-interacting serine-arginine-rich protein 1 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC001107 |
| 基因名称 | FUSIP1 |
| Gene ID (NCBI) | 10772 |
| RRID | AB_2877726 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75494 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUSIP1, also named as SRS10, or TASR, is a 262 amino acid protein, which belongs to the splicing factor SR family. FUSIP1 as a Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. FUSIP1 is required for splicing repression in M-phase cells and after heat shock and also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. FUSIP1 Interacting with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUSIP1/SRS10 antibody 10131-1-AP | Download protocol |
| IHC protocol for FUSIP1/SRS10 antibody 10131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. | ||
J Exp Clin Cancer Res SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway.
| ||
Proteomics Clin Appl Integration of the cancer cell secretome and transcriptome reveals potential noninvasive diagnostic markers for bladder cancer |
