IHCeasy SRSF10 Ready-To-Use IHC Kit

SRSF10 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1475

Synonyms

40 kDa SR repressor protein, FUSIP1, FUSIP2, NSSR, SFRS13, SFRS13A, Splicing factor SRp38, SRp38, SRrp40, SRSF10, TASR, TASR1, TASR2, TLS associated SR protein



Product Information

KHC1475 is a ready-to-use IHC kit for staining of SRSF10. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy SRSF10 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

FUSIP1, also named as SRSF10, SRrp40, SRp38 ,FUSIP2, SFRS13A, or TASR, is a 262 amino acid protein, which belongs to the splicing factor SR family. FUSIP1 as a Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. FUSIP1 is required for splicing repression in M-phase cells and after heat shock and also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. FUSIP1 Interacting with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms 40 kDa SR repressor protein, FUSIP1, FUSIP2, NSSR, SFRS13, SFRS13A, Splicing factor SRp38, SRp38, SRrp40, SRSF10, TASR, TASR1, TASR2, TLS associated SR protein