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验证数据展示

  • WB analysis of Jurkat using 22141-1-AP

    WB analysis of Jurkat using 22141-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 22141-1-AP

    IF Staining of A431 using 22141-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FUT4 antibody (22141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 22141-1-AP

    IHC staining of human lung cancer using 22141-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human lung cancer using 22141-1-AP

    IHC staining of human lung cancer using 22141-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 40x lens).

FUT4 Polyclonal antibody

FUT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22141-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, Alpha-(1,3)-fucosyltransferase 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A431 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22141-1-AP targets FUT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FUT4 fusion protein Ag17565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
别名 FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, Alpha-(1,3)-fucosyltransferase 4
计算分子量 530 aa, 59 kDa
观测分子量 45 kDa, 63 kDa
GenBank蛋白编号BC136374
基因名称 FUT4
Gene ID (NCBI) 2526
RRIDAB_2879006
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22083
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 22141-1-AP detects the band around 45 kDa and glycosylated isoform 63 kDa protein in SDS-PAGE. (PMID: 22287018, 17335083, 11278338)

实验方案

Product Specific Protocols
WB protocol for FUT4 antibody 22141-1-APDownload protocol
IHC protocol for FUT4 antibody 22141-1-APDownload protocol
IF protocol for FUT4 antibody 22141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols