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验证数据展示

  • IF Staining of A431 using CL488-22141

    IF Staining of A431 using CL488-22141

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 FUT4 antibody (CL488-22141) at dilution of 1:200.

CoraLite® Plus 488-conjugated FUT4 Polyclonal antibody

FUT4 Polyclonal Antibody for IF/ICC
Cat No. CL488-22141

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Alpha (1,3) fucosyltransferase, CD15, ELFT, FCT3A, FUC TIV, Fucosyltransferase 4, Fucosyltransferase IV, FucT IV, FUT4, FUTIV, SSEA 1, SSEA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 22141-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22141 targets FUT4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 FUT4 fusion protein Ag17565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
别名 Alpha (1,3) fucosyltransferase, CD15, ELFT, FCT3A, FUC TIV, Fucosyltransferase 4, Fucosyltransferase IV, FucT IV, FUT4, FUTIV, SSEA 1, SSEA1
计算分子量 530 aa, 59 kDa
观测分子量 45 kDa, 63 kDa
GenBank蛋白编号BC136374
基因名称 FUT4
Gene ID (NCBI) 2526
RRIDAB_3084062
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22083
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 22141-1-AP detects the band around 45 kDa and glycosylated isoform 63 kDa protein in SDS-PAGE. (PMID: 22287018, 17335083, 11278338)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FUT4 antibody CL488-22141Download protocol
Standard Protocols
Click here to view our Standard Protocols