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验证数据展示

  • IF Staining of mouse brain using CL647-67813

    IF Staining of mouse brain using CL647-67813

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 FXR1 antibody (CL647-67813, Clone: 1E12E8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL647-67813

    FC experiment of HeLa using CL647-67813

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human FXR1 (CL647-67813, Clone:1E12E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated FXR1 Monoclonal antibody

FXR1 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-67813

产品说明书

CloneNo. 1E12E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FXR1 Ready-To-Use IHC Kit. FXR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67813-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67813 targets FXR1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 FXR1 fusion protein Ag27800 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fragile X mental retardation, autosomal homolog 1
别名 RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8
计算分子量 621 aa, 70 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC028983
基因名称 FXR1
Gene ID (NCBI) 8087
RRIDAB_2920315
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP51114
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MV 69 kDa and 60 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 FXR1 antibody CL647-67813Download protocol
FC protocol for CL Plus 647 FXR1 antibody CL647-67813Download protocol
Standard Protocols
Click here to view our Standard Protocols