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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated FXR1 Monoclonal antibody

FXR1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E12E8

Cat no : CL647-67813

Print datasheet

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

验证数据展示

  • IF Staining of mouse brain using CL647-67813

    IF Staining of mouse brain using CL647-67813

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 FXR1 antibody (CL647-67813, Clone: 1E12E8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL647-67813

    FC experiment of HeLa using CL647-67813

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human FXR1 (CL647-67813, Clone:1E12E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FXR1 Ready-To-Use IHC Kit. FXR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67813-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67813 targets FXR1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen FXR1 fusion protein Ag27800 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fragile X mental retardation, autosomal homolog 1
Synonyms RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8
Calculated Molecular Weight 621 aa, 70 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC028983
Gene Symbol FXR1
Gene ID (NCBI) 8087
RRIDAB_2920315
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51114
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MV 69 kDa and 60 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 FXR1 antibody CL647-67813Download protocol
FC protocol for CL Plus 647 FXR1 antibody CL647-67813Download protocol
Standard Protocols
Click here to view our Standard Protocols