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验证数据展示

  • FC experiment of C2C12 using CL594-67150

    FC experiment of C2C12 using CL594-67150

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human FXYD1 (CL594-67150, Clone:2C10G9) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67150

产品说明书

CloneNo. 2C10G9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Pig, mouse

应用

FC (Intra)

别名

FXYD1, Phospholemman, PLM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inC2C12 cells
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy FXYD1 Ready-To-Use IHC Kit. FXYD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67150-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67150 targets FXYD1 in FC (Intra) applications and shows reactivity with Human, Pig, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Pig, mouse
免疫原 FXYD1 fusion protein Ag25307 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 1
别名 FXYD1, Phospholemman, PLM
计算分子量 92 aa, 10 kDa
观测分子量 15 kDa
GenBank蛋白编号BC032800
基因名称 FXYD1
Gene ID (NCBI) 5348
RRIDAB_3084754
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00168
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

实验方案

Product Specific Protocols
FC protocol for CL594 FXYD1 antibody CL594-67150Download protocol
Standard Protocols
Click here to view our Standard Protocols