Frizzled 10 Polyclonal antibody

Frizzled 10 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 18175-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF-P, ELISA and More (1)

FZD10, FzE7, Fz-10, FZ 10, Frizzled-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18175-1-AP targets Frizzled 10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, chicken, zebrafish, goat
免疫原 Frizzled 10 fusion protein Ag12867 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 frizzled homolog 10 (Drosophila)
别名 FZD10, FzE7, Fz-10, FZ 10, Frizzled-10
计算分子量 581 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC074998
基因名称 Frizzled 10
Gene ID (NCBI) 11211
RRIDAB_2108942
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULW2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.

实验方案

Product Specific Protocols
WB protocol for Frizzled 10 antibody 18175-1-APDownload protocol
IHC protocol for Frizzled 10 antibody 18175-1-APDownload protocol
IF protocol for Frizzled 10 antibody 18175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,FC

Nat Commun

Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.

Authors - Alison E Jarmas
chickenIHC,IF

Development

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
zebrafishWB

Development

Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle.

Authors - Alissa Caron
humanWB

Oncotarget

Non coding RNA analysis in fibrolamellar hepatocellular carcinoma.

Authors - Benjamin A Farber
humanWB

Int J Oncol

Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.

Authors - Qian Sun
goatIHC

Cells

Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.

Authors - Shanhe Wang
Loading...