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Fc epsilon RI alpha Recombinant antibody

Fc epsilon RI alpha Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241848D1

Cat no : 84662-3-RR

Print datasheet

Synonyms

FCER1A, FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A

验证数据展示

  • WB analysis using 84662-3-RR

    WB analysis using 84662-3-RR

    Human testis was subjected to SDS PAGE followed by western blot with 84662-3-RR (Fc epsilon RI alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84662-3-RR targets Fc epsilon RI alpha in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
Synonyms FCER1A, FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A
Calculated Molecular Weight30kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberNM_001387280.1
Gene Symbol Fc Epsilon RI Alpha
Gene ID (NCBI) 2205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, also known as high affinity immunoglobulin epsilon receptor subunit alpha, FCER1A and FCE1A, is a single-pass type I membrane protein which contains 2 immunoglobulin-like domains. FCER1A is a subunit of the IgE receptor, which is composed of one glycosylated alpha (FCER1A), one beta (FCER1B), and two gamma (FCER1G) subunits. The high affinity IgE receptor plays a central role in allergic disease, coupling allergen and mast cells to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The calculated molecular weight of FCER1A is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).

实验方案

Product Specific Protocols
WB protocol for Fc epsilon RI alpha antibody 84662-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols