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FcRn Monoclonal antibody

FcRn Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4A5

Cat no : 67944-1-Ig

Print datasheet

Synonyms

alpha chain, FCGRT, FCRN, Neonatal Fc receptor

验证数据展示

  • WB analysis using 67944-1-Ig

    WB analysis using 67944-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67944-1-Ig (FcRn antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67944-1-Ig

    IHC staining of human liver using 67944-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67944-1-Ig (FcRn antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67944-1-Ig

    IHC staining of human liver using 67944-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67944-1-Ig (FcRn antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67944-1-Ig targets FcRn in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen FcRn fusion protein Ag31173 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgG, receptor, transporter, alpha
Synonyms alpha chain, FCGRT, FCRN, Neonatal Fc receptor
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC008734
Gene Symbol FcRn
Gene ID (NCBI) 2217
RRIDAB_2918696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55899
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).

实验方案

Product Specific Protocols
WB protocol for FcRn antibody 67944-1-IgDownload protocol
IHC protocol for FcRn antibody 67944-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols