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验证数据展示

  • WB analysis using 67944-1-Ig

    WB analysis using 67944-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67944-1-Ig (FcRn antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67944-1-Ig

    IHC staining of human liver using 67944-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67944-1-Ig (FcRn antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67944-1-Ig

    IHC staining of human liver using 67944-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67944-1-Ig (FcRn antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FcRn Monoclonal antibody

FcRn Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67944-1-Ig

产品说明书

CloneNo. 2H4A5

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

alpha chain, FCGRT, FCRN, Neonatal Fc receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67944-1-Ig targets FcRn in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 FcRn fusion protein Ag31173 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, receptor, transporter, alpha
别名 alpha chain, FCGRT, FCRN, Neonatal Fc receptor
计算分子量 40 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC008734
基因名称 FcRn
Gene ID (NCBI) 2217
RRIDAB_2918696
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP55899
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).

实验方案

Product Specific Protocols
WB protocol for FcRn antibody 67944-1-IgDownload protocol
IHC protocol for FcRn antibody 67944-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols