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Fibrillin 1 Polyclonal antibody, PBS Only

Fibrillin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29425-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 29425-1-AP (same clone as 29425-1-PBS)

    WB analysis using 29425-1-AP (same clone as 29425-1-PBS)

    HeLa cells were subjected to Tris-acetate gel system followed by western blot with 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

    IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 29425-1-AP (same clone as 29425-1-PBS)

    IF Staining of A431 using 29425-1-AP (same clone as 29425-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Fibrillin 1 antibody (29425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 29425-1-AP (same clone as 29425-1-PBS)

    IHC staining of human colon using 29425-1-AP (same clone as 29425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

    IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

产品信息

29425-1-PBS targets Fibrillin 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fibrillin 1 fusion protein Ag30283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibrillin 1
Synonyms
Observed Molecular Weight350 kDa
GenBank Accession NumberBC146854
Gene Symbol Fibrillin 1
Gene ID (NCBI) 2200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35555
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).