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验证数据展示

  • IF Staining of HepG2 using CL488-11867

    IF Staining of HepG2 using CL488-11867

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Ficolin-3 antibody (CL488-11867) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Ficolin-3 Polyclonal antibody

Ficolin-3 Polyclonal Antibody for IF/ICC
Cat No. CL488-11867

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

FCN3, FCNH, Ficolin 3, Ficolin-3, HAKA1, Hakata antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11867-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11867 targets Ficolin-3 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Ficolin-3 fusion protein Ag2449 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
别名 FCN3, FCNH, Ficolin 3, Ficolin-3, HAKA1, Hakata antigen
计算分子量 33 kDa
观测分子量 32 kDa
GenBank蛋白编号BC020731
基因名称 Ficolin-3
Gene ID (NCBI) 8547
RRIDAB_3672516
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75636
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains (PMID: 20375620). They are innate pattern recognition receptors and play integral roles within the innate immune response. In humans, there are three ficolins termed M-, L-, and H-ficolin (also referred to as ficolin-1, -2, and -3) whereas rodents only possess ficolin-A and -B, which are the orthologues of human L- and M-ficolin, respectively (PMID: 30868077). Ficolin-3 (Hakata antigen or H-ficolin) was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus (PMID: 1859827). It has been shown to have a calcium-independent lectin activity. Ficolin-3 is associated with MASPs and sMAP, and which activates the lectin pathway (PMID: 11907111). Ficolin-3 shows affinity for GalNAc, GlcNAc, D-fucose and galactose (PMID: 30868077).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Ficolin-3 antibody CL488-11867Download protocol
Standard Protocols
Click here to view our Standard Protocols