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验证数据展示

  • IF Staining of HeLa using 85202-1-RR

    IF Staining of HeLa using 85202-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 85202-1-RR

    IF Staining of A549 using 85202-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293T using 85202-1-RR

    FC experiment of HEK-293T using 85202-1-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Filamin B Recombinant antibody (85202-1-RR, Clone:242275B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Filamin B Recombinant antibody

Filamin B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85202-1-RR

产品说明书

CloneNo. 242275B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, A549 cells
Positive FC (Intra) detected inHEK-293T cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85202-1-RR targets Filamin B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 filamin B, beta (actin binding protein 278)
别名 FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog
计算分子量 278 kDa
GenBank蛋白编号NM_001457
基因名称 FLNB
Gene ID (NCBI) 2317
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75369
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis.

实验方案

Product Specific Protocols
IF protocol for Filamin B antibody 85202-1-RRDownload protocol
FC protocol for Filamin B antibody 85202-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols