验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with Mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 文献引用应用 | FC (Intra) |
| 经测试反应性 | Mouse |
| 文献引用反应性 | mouse |
| 免疫原 | N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box P3 |
| 别名 | forkhead box P3, Foxp3, JM2, scurfin, sf |
| GenBank蛋白编号 | BC132333 |
| 基因名称 | Foxp3 |
| Gene ID (NCBI) | 20371 |
| RRID | AB_2919258 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Foxp3 antibody CL488-65089 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |