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FITC Anti-Mouse Foxp3 (3G3)

Foxp3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Mouse

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

3G3

Cat no : FITC-65089

Print datasheet

Synonyms

forkhead box P3, Foxp3, JM2, scurfin, sf

验证数据展示

  • FC experiment of mouse splenocytes using FITC-65089

    FC experiment of mouse splenocytes using FITC-65089

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and then treated with fixation and permeabilization reagents. Cells were then stained with 0.13 ug FITC Foxp3 antibody (FITC-65089, Clone: 3G3).

  • FC experiment of mouse splenocytes using FITC-65089

    FC experiment of mouse splenocytes using FITC-65089

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and then treated with fixation and permeabilization reagents. Cells were then stained with 0.13 ug FITC-mouse IgG1 isotype control antibody.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65089 targets Foxp3 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name forkhead box P3
Synonyms forkhead box P3, Foxp3, JM2, scurfin, sf
GenBank Accession NumberBC132333
Gene Symbol Foxp3
Gene ID (NCBI) 20371
RRIDAB_2883771
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths494 nm / 520 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ99JB6
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

实验方案

Product Specific Protocols
FC protocol for FITC Foxp3 antibody FITC-65089Download protocol
Standard Protocols
Click here to view our Standard Protocols