验证数据展示
经过测试的应用
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66486-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-66486 targets G3BP1 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | G3BP1 fusion protein Ag3728 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GTPase activating protein (SH3 domain) binding protein 1 |
| 别名 | G3BP, G3BP 1, G3BP1, HDH VIII |
| 计算分子量 | 466 aa, 52 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC006997 |
| 基因名称 | G3BP1 |
| Gene ID (NCBI) | 10146 |
| RRID | AB_2934675 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13283 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 G3BP1 antibody CL555-66486 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |