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验证数据展示

  • WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

    WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82080-4-RR (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

    IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-4-RR, Clone: 230275D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve of MP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Sandwich ELISA standard curve of MP00090-3

    Sandwich ELISA standard curve of MP00090-3

    Sandwich ELISA standard curve of MP00090-3, Human G3BP2 Recombinant Matched Antibody Pair - PBS only. 82080-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9355. 82080-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00090-3

    Sample test of MP00090-3

    The mean G3BP2 concentration was determined to be 8.91 ng/mL in A549 cell extract based on a 2.20 mg/mL extract load, 11.75 ng/mL in HeLa cell extract based on a 3.20 mg/mL extract load, 9.66 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 29.51 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

  • FC experiment of U2OS using 82080-4-RR (same clone as 82080-4-PBS)

    FC experiment of U2OS using 82080-4-RR (same clone as 82080-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-4-RR, Clone:230275D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP2 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP2 antibody included
  • Cytometric bead array standard curve of MP00090-1

    Cytometric bead array standard curve of MP00090-1

    Cytometric bead array standard curve of MP00090-1, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-3-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00090-2

    Cytometric bead array standard curve of MP00090-2

    Cytometric bead array standard curve of MP00090-2, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-5-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 82080-4-PBS

    Affinity and Kinetic Characterization of 82080-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 82080-4-PBS against Human G3BP2 was performed. The affinity constant is 3.48 nM.

  • Affinity and Kinetic Characterization of 82080-4-RR

    Affinity and Kinetic Characterization of 82080-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82080-4-RR against Human G3BP2 were performed. The affinity constant is 59.6 nM.

G3BP2 Recombinant antibody, PBS Only (Capture)

G3BP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82080-4-PBS

产品说明书

CloneNo. 230275D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

G3BP 2, 230275D6, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.

产品信息

82080-4-PBS targets G3BP2 as part of a matched antibody pair:

MP00090-1: 82080-4-PBS capture and 82080-3-PBS detection (validated in Cytometric bead array)

MP00090-2: 82080-4-PBS capture and 82080-5-PBS detection (validated in Cytometric bead array)

MP00090-3: 82080-4-PBS capture and 82080-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 G3BP2 fusion protein Ag9355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GTPase activating protein (SH3 domain) binding protein 2
别名 G3BP 2, 230275D6, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2
计算分子量482aa,54 kDa; 449aa,51 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC011731
基因名称 G3BP2
Gene ID (NCBI) 9908
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UN86
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.