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验证数据展示

  • IF Staining of HeLa using 82080-6-RR (same clone as 82080-6-PBS)

    IF Staining of HeLa using 82080-6-RR (same clone as 82080-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-6-RR, Clone: 230275G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

  • WB analysis using 82080-6-RR (same clone as 82080-6-PBS)

    WB analysis using 82080-6-RR (same clone as 82080-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82080-6-RR (G3BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82080-6-RR (same clone as 82080-6-PBS)

    WB analysis of HepG2 using 82080-6-RR (same clone as 82080-6-PBS)

    WB result of G3BP2 antibody (82080-6-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP2 transfected HepG2 cells. This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR (same clone as 82080-6-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR (same clone as 82080-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP2 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR (same clone as 82080-6-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR (same clone as 82080-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP2 antibody included
  • FC experiment of U2OS using 82080-6-RR (same clone as 82080-6-PBS)

    FC experiment of U2OS using 82080-6-RR (same clone as 82080-6-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-6-RR, Clone:230275G3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82080-6-RR

    Affinity and Kinetic Characterization of 82080-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82080-6-RR against Human G3BP2 were performed. The affinity constant is 4.69 nM.

  • Featured Product
  • KD/KO Validated

G3BP2 Recombinant antibody, PBS Only

G3BP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82080-6-PBS

Print datasheet

CloneNo. 230275G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

G3BP 2, 230275G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.

产品信息

82080-6-PBS targets G3BP2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen G3BP2 fusion protein Ag9355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 2
Synonyms G3BP 2, 230275G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2
Calculated Molecular Weight482aa,54 kDa; 449aa,51 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC011731
Gene Symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UN86
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.