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验证数据展示

  • WB analysis using 66860-1-Ig (same clone as 66860-1-PBS)

    WB analysis using 66860-1-Ig (same clone as 66860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 66860-1-Ig (same clone as 66860-1-PBS)

    WB analysis of HuH-7 using 66860-1-Ig (same clone as 66860-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50826-2

    Cytometric bead array standard curve of MP50826-2

    Cytometric bead array standard curve of MP50826-2, G6PC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66860-4-PBS. Detection antibody: 66860-1-PBS. Standard:Ag17839. Range: 0.098-100 ng/mL.

G6PC Monoclonal antibody, PBS Only (Detector)

G6PC Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66860-1-PBS

产品说明书

CloneNo. 1E11A5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

G6PC1, G6Pase-alpha, G-6-Pase, G6Pase, EC:3.1.3.9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66860-1-PBS targets G6PC as part of a matched antibody pair:

MP50826-2: 66860-4-PBS capture and 66860-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 G6PC fusion protein Ag17839 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glucose-6-phosphatase, catalytic subunit
别名 G6PC1, G6Pase-alpha, G-6-Pase, G6Pase, EC:3.1.3.9
计算分子量 357 aa, 40 kDa
观测分子量 37-42 kDa
GenBank蛋白编号BC130478
基因名称 G6PC
Gene ID (NCBI) 2538
RRIDAB_2882199
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35575
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).