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  • Featured Product
  • KD/KO Validated

G6PD Monoclonal antibody, PBS Only

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B12

Cat no : 66373-1-PBS

Print datasheet

Synonyms

G6P, G6PD, G6PD1

验证数据展示

  • WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

    WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

    Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G6PD antibody included
  • IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G6PD antibody included
  • IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66373-1-Ig(G6PD antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.

产品信息

66373-1-PBS targets G6PD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen G6PD fusion protein Ag21862 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glucose-6-phosphate dehydrogenase
Synonyms G6P, G6PD, G6PD1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000337
Gene Symbol G6PD
Gene ID (NCBI) 2539
RRIDAB_2877122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11413
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.