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  • KD/KO Validated

G6PD Monoclonal antibody

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2A7B12

Cat no : 66373-1-Ig

Print datasheet

Synonyms

G6PD, G6PD1



经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, rat spleen tissue, L02 cells, HEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66373-1-Ig targets G6PD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen G6PD fusion protein Ag21862 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glucose-6-phosphate dehydrogenase
Synonyms G6PD, G6PD1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000337
Gene Symbol G6PD
Gene ID (NCBI) 2539
RRIDAB_2877122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11413
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for G6PD antibody 66373-1-IgDownload protocol
IHC protocol for G6PD antibody 66373-1-IgDownload protocol
IF protocol for G6PD antibody 66373-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIHC,IF,IP

Cell Metab

Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.

Authors - Yang Zhang
mouse,ratWB

Redox Biol

The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.

Authors - Shenglan Zeng
human,mouseWB,IHC

Cell Rep

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation

Authors - Zehe Ge
mouseWB

Clin Transl Med

SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.

Authors - Tiantong Ou
mouseWB

Int J Mol Sci

G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.

Authors - Aiwen Jiang
  • KD Validated
humanWB

J Pharmacol Exp Ther

Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.

Authors - Ramon Martinez